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Precision analysis of urinary micro-albumin on conventional Hitachi 912 and modular Cobas 6000 C501 analyzers

Author: 
Junaid Mahmood Alam, Humaira (Howrah) Ali, Jawed Altaf Baig, Aijaz Ahmed and Sheikh Matinuddin
Subject Area: 
Life Sciences
Abstract: 

Background: Clinical significance of urinary micro-albumin in diabetes, cardiovascular disease and hypertension generated interest amongst scientist, researchers and clinicians to enhance its measurement accuracy and level of precision. Aim and Objectives: Present study describes the comparative precision analysis of urinary micro-albumin on conventional Hitachi 912 chemistry analyzer and modular Cobas 6000 c501 system. Material and Methods: A total 240 patients (Males = 120, Females = 120), n = 40 for each disease (Diabetes, Hypertension, Renal disease) in both genders, were selected for the present study during Dec 2013 to Dec 2014. Determination of micro-albumin was performed in 2nd morning urine collected from all patients and co-morbid groups and analyzed in duplicate using TINA-QUANT albumin methodology (Roche Diagnostics). Results: Comparative precision analyses data showed 93% to 97% correlation of analytical precisions among both conventional Hitachi and Cobas modular systems, thus guaranteeing equitable quality assurance of methods and instrumentations. Conclusion: It was concluded that 93% to 97% linear linked precision existed in analytical steps of conventional and modular system with R2 0.93 to 0.97 in various clinical groups of patients, suggesting considerable unbiased accurateness.

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