A study has been undertaken to optimize the amylolytic activity of Streptomyces tendae TK-VL_333 isolated from laterite soil samples of Guntur region by using basal medium as inorganic salts-starch medium. Four-day old culture showed maximum enzymatic activity when grown at pH 7.0 and temperature 30oC. The productivity of the strain was enhanced by amending the medium with sorghum flour and peptone at a concentration of 3% (w/v) and 0.5% (w/v) respectively. This is the first report on the production of amylases by S. tendae.