Dengue, a mosquito-transmitted viral disease that produces variable symptoms, ranging from asymptomatic infection to life-threatening disease, is present in about 110 tropical and subtropical countries. As dengue is increasing in incidence, improved diagnosis, early detection of severe cases, and efficient medical management are of primary importance in all areas where dengue is endemic. Traditionally, dengue has been diagnosed by virus isolation or serological methods, but with recent advances in molecular techniques and in rapid detection technology, a range of novel diagnostic tests will soon be commercially available that will improve case management and aid disease control efforts.