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Role of metabolism of hdl and its subtypes in occurrence of diabetes in off springs of patients with type 2 diabetes mellitus

Author: 
Sumayya Wani, Ishrat H. Dar, Ambreen Bashir, Sair B. Mir and Madan L. Khurana
Subject Area: 
Health Sciences
Abstract: 

Background: Patients with Diabetes mellitus (DM) develop low levels of High Density Lipoprotein (HDL) during the initial progression of disease. There is growing appreciation that Insulin resistance is associated with lowering of HDL. The exact prevalence of the Insulin resistance syndrome among those with low HDL in the general population has not been clearly established. Methods: One twenty one patients and one twenty one controls were taken. Precipitation reagent (0.06 ml) containing 1071 U/ml Heparin, 500 mmol/l Magnesium Chloride and 12 mg/ml Dextran Sulfate was added to a Serum (0.3 ml). Sample was incubated and centrifuged at 10,000 rpm for 10 minutes. HDL3 was measured by a homogenous HDL assay in the supernatant and HDL2 estimated by subtracting the HDL3 from direct HDL. Results: Mean HDL of patients and controls was 35.74 ± 8.18 and 49.27 ± 37.28 respectively. HDL3 was 23.84 ± 5.97 and 31.08 ± 4.60 respectively.HDL2 resulted18.27± 5.55 and 11.84 ± 3.61 respectively. Mean Insulin levels were 11.33 ± 8.46 and 9.14 ± 5.60 respectively. Conclusions: HDL2 is higher than HDL3in off springs of patients with Type 2 DM.HenceHDL2 may serve as an initial marker for Type 2 DM

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