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Screening and identification of human rotavirus by elisa and rna-page method

Author: 
Amit Kumar Yadav, Hari Mohan Saini and Sandeep Khobrai
Subject Area: 
Health Sciences
Abstract: 

Rotavirus is causative agent of diarrhea resulting in mortality in children up to the age group of 5 years. The first infection occur between age group of 6-9 months. However, the need for screening of rotavirus is important to access the incidence of severe disease or effectiveness and its serotype distributions. To detect any new ideotype in re-assortment a continuos screening of existence genotype is required. Multiple assay now exist for measuring the serological response to Rotavirus infection. ELISA and RNA-PAGE are considered to be highly sensitive tool for screening of Rotavirus because its abilty to detect even at low concentration.

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