Selective genotyping for determining the linkage between SSR markers and a fertility restoration locus in Sorghum bicolor (L.) Moench

Cytoplasmic male sterility (CMS) is a genetic condition in which the male sterile plants are not able to set seeds on their own. Both cytoplasmic and nuclear genes interact to control male sterility. Restorer of fertility (Rf) genes restore male fertility on CMS plants. In the present study, we genetically mapped a fertility restoration locus using selective genotyping approach in a F2 population of 296A x PVK809 cross. The Rf locus was identified near to the SSR marker Xtxp304 on chromosome 2. Sorghum gene, Sobic.002G057000, a member of Pentatricopeptide (PPR) protein family was putatively identified as the candidate for fertility restoration in this cross.