The present study was the humic acid (HA)-oxidizing and the reduced organisms isolated from different localities of sewage samples in and around the 12 district of Tamil Nadu. The additional electron donating capacity of reduced HA could reasonably be attributed to the oxidation of reduced functional groups. Furthermore, this study indicates that reduced humic acids impact soil geochemistry and the indigenous bacterial community, on the basis of α, β, γ, and σ – Proteobacteria were includes heterotrophs, autotrophs, and methanotrophs. Among them methanotrophic sulfur reducing were isolated and screened by humic acid supplementation under the anaerobic fermentation. Signal transduction of ATP synthase by H2 electron donor and acceptor, the donation H2 molecule during the cell division by inducing the activity of metabolic enzyme binding and non-binding site of the cell wall in the presence of humic acid. However, further studies of metabolic interaction of the bacterial cell under humic acid supplementation traumatically changed in bacterial growth and morphology of the color have become a typical blue/greenish florescent in distinctly appear