CALL FOR PAPERS

Author Information

CERTIFICATE

IMPACT FACTOR 2019

Subject Area

Life Sciences / Biology
Architecture / Building Management
Asian Studies
Business & Management
Chemistry
Computer Science
Economics & Finance
Engineering / Acoustics
Environmental Science
Agricultural Sciences
Pharmaceutical Sciences
General Sciences
Materials Science
Mathematics
Medicine
Nanotechnology & Nanoscience
Nonlinear Science
Chaos & Dynamical Systems
Physics
Social Sciences & Humanities

Why Us? >>

Open Access
Peer Reviewed
Rapid Publication
Life time hosting
Free promotion service
Free indexing service
More citations
Search engine friendly

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

Standardization of one-step single tube rt-pcr for rapid detection of all circulating strains of dengue viruses

Author:

Sulochana Kaushik, Vikrant Sharma, Dhruva Chaudhary, Sanjeev Nanda, Jaya Parkash Yadav and Samander Kaushik

Subject Area:

Life Sciences

Abstract:

Dengue is a viral disease which is spread by a mosquito vector and is prevalent in tropical and subtropical regions. Dengue poses serious health threat to a major portion of population therefore rapid and specific tests are needed to combat the situation. Thus, one-step reverse transcription polymerase chain reaction (RT-PCR) was standardized to detect all the four serotypes of dengue viruses i.e. DENV1 to DENV4. This study was performed on standard strains of dengue viruses. RNA was extracted from these strains and was used to optimize one step RT-PCR. This assay was able to detect all the four serotypes of dengue virus.

PDF file: