The present study was intended to determine the ovarian developmental stages and changes in the level of vitellogenin and vitellin as a biomarker of ovary maturation. Five different stages were classified based on the size and colour of the ovary. Histological studies clearly illustrate a gradual increase in oocyte development from stage I to IV based on yolk accumulation. Enzyme linked immunosorbent assay results also further confirmed gradual increase in the level of Vitellogenin and vitellin levels as the ovary maturation proceeds. The present study was intended to determine the ovarian developmental stages and changes in the level of vitellogenin and vitellin as a biomarker of ovary maturation. Five different stages were classified based on the size and colour of the ovary. Histological studies clearly illustrate a gradual increase in oocyte development from stage I to IV based on yolk accumulation. Enzyme linked immunosorbent assay results also further confirmed gradual increase in the level of Vitellogenin and vitellin levels as the ovary maturation proceeds.