Study of secondary metabolites from cell suspension of gymnema sylvestre (retz.) r. br. ex. schult. by using elicitors

The present study aimed to explain the effect of biotic and abiotic elicitor derived from yeast extract and Salicylic acid on cell suspensions evolved from superior callus lines of G. sylvestre (Retz) R. Br. ex. Schult. Cell suspension cultures maintained in MS liquid medium were treated with 265 cells/ml and 530 cells/ml homogenate of the elicitor on the 14th day. Growth of G. sylvestre callus in terms of biomass revealed 5 fold enhancements when treated with 530 cells/ml. elicitor on fourteenth day in callus line-1. The type of callus line and concentration of the elicitor influence the biomass growth of G. sylvestre callus in suspension culture and gymnemic acid production was accomplish with the help of biotic and abiotic elicitor treatment. (as confirmed by HPTLC analysis)