TDZ induced in vitro plant regeneration of Momordica balsamina and Momordica charantia, important medicinal cucurbits

The comparative study was conducted to analyze the effect of TDZ on Caullogenesis of M. balsamina and M. charantia a closely related important medicinal cucurbits. The highest number of (10.9 ± 0.05) multiple shoots were observed on MS medium fortified with TDZ+ CSH (2.0 + 200 mg/L) from cotyledonary explant of M. balsamina However cotyledonary explants were produced maximum number of shoots (9.00± 0.23) on MS medium augmented with TDZ + CSH (2.0 + 200 mg/L) in M. charantia. Best rooting response obtained from regenerated shoots on MS medium supplemented with IBA (3.0 mg/L) in both plants. The plantlets raised in vitro were transferred in to green house and successfully acclimatized to natural condition with 75 % survival rate. This study is used for the analysis of effect of various concentration of TDZ on both plants and it is tool for genetics and transformation applications.