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TDZ induced in vitro plant regeneration of Momordica balsamina and Momordica charantia, important medicinal cucurbits

Author:

Peer Mahammad Shaik, Raju Samraju, Ravi Chithakari and Mustafa Mohammad

Subject Area:

Life Sciences

Abstract:

The comparative study was conducted to analyze the effect of TDZ on Caullogenesis of M. balsamina and M. charantia a closely related important medicinal cucurbits. The highest number of (10.9 ± 0.05) multiple shoots were observed on MS medium fortified with TDZ+ CSH (2.0 + 200 mg/L) from cotyledonary explant of M. balsamina However cotyledonary explants were produced maximum number of shoots (9.00± 0.23) on MS medium augmented with TDZ + CSH (2.0 + 200 mg/L) in M. charantia. Best rooting response obtained from regenerated shoots on MS medium supplemented with IBA (3.0 mg/L) in both plants. The plantlets raised in vitro were transferred in to green house and successfully acclimatized to natural condition with 75 % survival rate. This study is used for the analysis of effect of various concentration of TDZ on both plants and it is tool for genetics and transformation applications.

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