Total phenolic, total flavonoid content and antioxidant activity of leaves extracts of Chenopodium album L. and Atriplex hortensis L.

The current investigation was conducted on Chenopodium album L. and Atriplex hortensis L. to estimate the contents of total phenolics, total flavonoids and antioxidant activity by using four different solvent extracts. Total phenolic content was determined by using the Folin-Ciocalteu method while total flavonoid contents were estimated by using Aluminium chloride (AlCl3) colorimetric assay. The antioxidant avtivity in the form ABTS 2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) was evaluated with the help of spectrophotometric method. Among all the tested extracts the highest amounts of total phenolic content was observed in A. hortesnsis methanolic extract 32.33±0.021 gallic acid equivalent (GAE)/g. Similarly the highest flavonoid content was observed in A. hortesnsis ethanolic extract 1.75±0.026 mg of quercetin equivalent/g. The highest ABTS free radical scavenging activity was observed in A. hortesnsis ethyle acetate extract 98.6 %±0.015μg/ml.