A valuable Actinomycete strain with antagonistic activity was isolated, identified and confirmed as Nocadiopsis sp. based on the 16S rRNA sequence analysis and biochemical examinations. Strain JAJ16 was subjected to cultural characterization with respect to aerial mycelium color, diffusible pigments production and the growth characteristics on different media. Extensive growth with bactericidal compound secretion was observed in ISP4 with 5 % (w/v) NaCl. Antimicrobial potential was screened against a list of bacteria and fungi. The strain JAJ16 showed good antimicrobial activity with significant minimum inhibitory concentration (MIC).