In vitro determination of marine sponge hyrtios erectus secondary metabolite effect against human breast and larynx cancer cell lines

Marine sponges are rich sources of novel secondary metabolite and they are potential drug molecule to antitumor and antiprolific drug development. Hyrtios erectus was extracted and purified through different solvent fractions methods. Pure compound was obtained as brownish amorphous powder. The positive test for dragendorff reagent was indicated as alkaloid group. Cytotoxicity was tested on Normal (Vero) cell line non tumor cells, Human Breast cancer cell line (MCF-7) and Human epithelial larynx cancer cell line (Hep-2) using microculture tetrazolium (MTT) assay for anticancer activity. Sponge pure compound was non‐toxic to (Vero) cells but highly cytotoxicity to (53%) MCF-7 cells and low toxic to Hep-2(32%) was recorded at 25μg/ml concentration. Each concentrations express individual results of cells viability with cytotoxicity increase and decrease of their concentration level of pure compound. In this research further investigation require for this sponge purified compound in order to chemical structure elucidations as well as pre clinical study for anticancer activity.