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In vitro multiplication of rose (rosa bourboniana)

Author:

Murali, R. and Sindhu, K

Subject Area:

Life Sciences

Abstract:

A protocol for in vitro multiplication of red rose (Rosa bourboniania) was developed. Multiple shoots were induced from nodal segments and shoot tips. MS (Murashige and Skoog) medium supplemented with various combinations of BAP (6-Benzyl Amino Purine), NAA (1-Naphthylene Acetic Acid), IAA ( Indole Acetic Acid) were used to study the in vitro growth response. Culture initiation media were used for further multiplication. MS medium incorporated with 2.0 mgl-1 BAP, 30 mgl-1, Ad.SO4 (Adenine sulphate) and 3% sucrose was found to be the most suitable medium for culture initiation and multiplication. Growth regulator combinations did not seem to influence multiple shooting and further growth in this species.

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