Roots of Chlorophytum borivilianum (safed musli) were regenerated by taking explants from isolated field grow mature plants. Two types explants stem disc and shoot base were used in the micropropagation. This was achieved by culturing on MS solid medium with and without harmones. Both leaf base and tuber explants gave very poor response. MS medium containing 3mg/l NAA gave more number of roots. The root proliferation was observed on full strength of MS medium after inoculation with the increasing concentration (1 to 3 mg/ml) for Indole acetic acid (IAA) at 1mg/l and Napthaline acetic acid (NAA) at 3mg/l. Micropropagation is to be the effective method compared with other methods of multiplication. This protocol can be used to generate cost-effective protocol for large scale in vitro cultivation of safed musli.
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