Working women are burdened with work and family commitments. Most of working women feel difficult in balancing their work and family life. In Health Care Sector, the employees have to perform shift duties. The nurses have to perform morning, evening and night shifts. The nurses at night shifts observe more stress than others; this shows a negative impact on their professional and personal lives. Research indicates that improper work scheduling and long working hours tend to cause adverse effect on employee’s health and well-being. This article highlights the issues connected to work life balance of female nurses and the factors that determine stress in work life balance of nurses working in Rajiv Gandhi Institute of Medical Sciences (RIMS), Kadapa district, Andhra Pradesh.