Detection and identification of Xylazine in blood sample of cattle during their illegal trafficking

A case of utilization of xylazine to induce sedation in cattle to avoid their noise and movement for illegal trafficking was reported. A veterinary tranquilizer xylazine was detected by high performance thin layer chromatography followed by Gas Chromatography - Mass spectrometer. The blood sample of cattle and used syringes was received by the laboratory from the scene of crime by investigating agency. The extraction of drug from the biological samples has been done by using liquid-liquid extraction method. The methods strongly support the presence of xylazine in sample of question and give firm evidence for the material used for the purpose of crime.