Effective in vitro propagation of medicinally valuable plant-Aristolochia Bracteolata, Lam

Innumerable plants were used in Indian Systems of medicine like Siddha, Ayurvedha and Unani. With increasing demand for herbal drugs, the natural populations of Aristolochia bracteolata are threatened with overexploitation. The present study was aimed to develop an innovative micropropagation protocol in Aristolochia bracteolata. Nodal and shoot tip explants were used as a starting material for micropropagation. Explants were surface sterilized and aseptically cultured on MS medium supplemented with different plant growth regulators. Best results of axillary bud formation were observed on MS medium containing 2.0 mg/l BAP. The multiple shoot formation from nodal segments were highest in MS medium supplemented with 1mg/l BAP + 2.0 mg/l kinetin. For rooting various concentrations of IBA were used. The rooted plantlets were finally hardened.