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Free radical scavenging activity of methanolic extract of diplazium esculentum- a wild fern of western ghats

Author: 
Archana G. Nair, Nikhila, G. S., Sangeetha, G. and Swapna, T. S.
Subject Area: 
Life Sciences
Abstract: 

An attempt was made to investigate the antioxidant activity of the methanolic extract of Diplazium esculentum. Antioxidant activity of D. esculentum was evaluated using DPPH (1, 1- Diphenyl -2- picrylhydrazyl) and Ferric reducing power along with superoxide, hydroxyl and nitric oxide radical scavenging assays. The methanol extract showed the most potent radical-scavenging activity in DPPH with 85.43 % at a concentration of 12.5µg/ml, whereas the reducing power of the standard ascorbic acid was found to be only 58.3 %. The IC50 value of the extract was found to be 25.72 μg/ml. The extract manifested significant reducing power (0.98) which exceeded even that of ascorbic acid (0.772) at a concentration of 200 μg/ml. At a maximum concentration of 200 μg/ml, the extract showed 62.4 % inhibition of hydroxyl radical, 51.8% inhibition of superoxide radicals and 87.6 % of nitric oxide radicals respectively. The present investigation suggest that the methanolic extract of D.esculentum has significant antioxidant activity, so can be used as a promising food supplement or it may be utilized as a potential source of therapeutic agent.

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