A protocol has been developed to facilitate regeneration and Agrobacterium mediated genetic transformation using antibiotics in regeneration of cotyledonary petiole and hypocotyls explants of Brassica juncea cv. NRCHB-101. Three antibiotics viz. Kanamycin (kan), Cefatoxime (Cef) and Hygromycin (hygro) have been used in this study during shoot regeneration along with the growth regulators. The antibiotic Cefatoxime overpowered the growth of Agrobacterium tumefaciens at minimum concentration (250 mg/l) whereas kanamycin and hygromycin not only eliminates the traces of excess Agrobacterium but also prevents the occurrence of false-positive shoots at the concentration of 20 mg/L. The present inquisition gives an account on the effectiveness of Kan, Cef and Hygro for regeneration and Agrobacterium tumefaciens-mediated genetic transformation in Brassica juncea cv. NRCHB-101.
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