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Preparation of 99mtc-ubi 29-41 complex samples under ultrasound irradiation technique

Author: 
Doroudi Alireza, Erfani Mostafa, Zaheri Maryam, Saadati Seyyed Mostafa, Ahmadi Faramarz, Kiasat Ali, Etesami Behrooz, Meghdadi Hossein and Rezaee Saeed
Subject Area: 
Health Sciences
Abstract: 

This study was launched to evaluate the efficacy of ultrasound irradiation technique versus boiling water bath method for preparation of 99mTc-UBI 29-41 samples and assess the bio distribution of radiotracer samples in the infected rats induced by Staphylococcus aureus. The 740MBq (20 mCi) 99mTc-UBI 29-41 samples were reconstituted by boiling water bath or ultrasound irradiation technique. The 99mTc-UBI 29-41 samples were administrated intravenously to the infected rats. Then qualitative and quantitative studies have been performed. The ITLC and radio-HPLC studies have demonstrated the 99mTc-UBI 29-41 samples with good yields could be prepared by new developed technique. All affected foot’s rats could be visualized by scintigraphy imaging. The imaging studies have indicated that the radiotracer samples prepared due to the above mentioned modalities shows very identical bio distribution in the infected rats. The ultrasound irradiation technique is convenient and efficient method to reconstitute the 99mTc-UBI 29-41 kits. It can be recommended as alternative method to prepare infection-seeking radiotracer samples in radioisotope imaging in the clinical practice.

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