Production and optimization of Lignocellulases by Aspergillus awamori in submerged fermentation

Aspergillus awamori is an efficient producer of many hydrolytic enzymes, has not been exploited commercially and for enzyme production. Cellulases, Xylanases and laccases thought to be of great significance for several industries namely paper, pharmaceuticals, food, feed etc. in addition to better utilization of lignocellulosic biomass. The present investigation was aimed to produce several extracellular enzymes by Aspergillus awamori and the Molecular characterization of the isolate by18S rRNA sequencing. Commercially available agricultural waste solid substrates were used as cellulosic substrates for enzyme production. Experiments were conducted to optimize the production of lignocellulases. The maximum enzyme activity was achieved on 6th day of incubation at 30O c temperature with the pH 6.0 and by using Lactose 1% w/v as Carbon Source, tryptone, yeast extract 1% w/v as Nitrogen Source. The present study reveals that the cellulolytic properties of Aspergillus awamori tested and optimized culture conditions maximizing lignocellulolytic enzymes production.