To study bacteriological profile and antimicrobial susceptibility pattern of pus isolates from maternal and child tertiary care hospital at western Rajasthan

Introduction: Pyogenic wound infections are responsible for causing significant morbidity resulting in longer duration of hospital stay and adding to economic burden for patient as well as hospital. The incidence of wound sepsis in India is reported to be 10-33%. Objectives: This study was done to identify bacteriological profile & their antimicrobial susceptibility pattern from various pus sample. Methods: This is retrospective study done in a tertiary care hospital from period of 1/4/22 to 31/3/23. In total 665 pus samples received from OBG and Pediatrics department were processed using standard microbiological methods and antibiotic sensitivity test was done as per CLSI 2022guidelines.Result:Out of 665 samples, 376 were culture positive (56.55%).Amongst them 350(93%) were females and 26(7%) were males. Maximum 42% cases in 21-30 year age group..Post LSCS wound infection cases were 227(60%) followed by puerperal sepsis (11.9%), post hysterectomy wound infection 30(7.97%).Gram positive cocci were predominant than Gram negative bacilli. 125 (33.2%) were CONS, 111(29.5%) were staphylococcus aureus, 57(15%) were Escherichia coli followed by Acinetobactersp 31(8.2%), Klebsiellasp 21(5.5%), Pseudomonas 18(4.7%). Staphylococcus aureus& Enterococcus species were 100% sensitive to linezolid and vancomycin. MRSA isolates were 62.16%. 86% of Escherichia coli were sensitive to gentamicin. Klebsiella pneumonia showed sensitivity to piperacillintazobactum (66.66%) and gentamicin (66.66%). Pseudomonas species & Acinetobacter were sensitive to aminoglycoside 88.88% & 70.96% respectively. Conclusion: Staphylococcus aureus and Escherichia coli were the most common causative bacteria isolated in this study with high resistance pattern. Studying the antibiogram of pus isolates in a setup can guide clinician to start appropriate empirical antibiotics and can escalate or deescalate as per culture and sensitivity report.